NATA
ecotox
SERVICES AUSTRALASIA
Toxicity Test Report: TR0294/1
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Client:
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Green Mountain Solutions
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ESA Job #:
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PR0294
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PO Box 1065
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Date Sampled:
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Not given
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Tweed Heads NSW 2485
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Date Received:
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13 September 2007
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Attention:
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Mr Steve Hall
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Sampled By:
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Green Mt Solutions
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Contract #:
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n/a
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Quote #:
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PI0294_q01
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Lab ID No.: 2320 Sample Name: Natra-Kleen Sample Description: Green liquid detergent
Test Performed:
Test Protocol: Deviations from Protocol: Source of Test Organisms: Test Initiated:
48-hr acute (survival) toxicity test using the freshwater cladoceran
Ceriodaphnia cfdubia
ESA SOP 101, based on USEPA (1993)
Nil
ESA Laboratory culture
3October2007at1115h
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Sample 2320: Natra-Kleen Concentration % Survival (mg/L) (at 24 hr)
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Sample 2320: Natra-Kleen Concentration % Survival (mg/L) (at 48 hr)
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0 (control) 100 ±0.0
6.25 100 ±0.0
12.5 100 ±0.0
25 100 ±0.0
50 75.0 ±19.2
100 0.0 ±0.0
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0 (control) 95.0 ±10.0
6.25 100 ±0.0
12.5 100 ±0.0
25 100 ±0.0
50 20.0 ± 0.0
100 0.0 ±0.0*
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24 hr EC50 = 59.5 (52.0-68.0)
mg/L
(TSK trim value = 0.0%)
NOEC = 50mg/L
LOEC = 100mg/L
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48 hr EC50 = 40.7 (35.9-46.1)
mg/L
(TSK trim value = Q.0%)
NOEC = 25mg/L
LOEC - 50mg/L
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"Significantly lower % survival compared with the control treatment (Steel's Many-One Rank Test, 1-tailed, P=0.05)
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QA/QC Parameter
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Criterion
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This Test
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Criterion met?
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Control % survival
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>90 %
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95.0%
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Yes
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Test Temperature limits
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25.0 ± 1 °C
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25.0°C
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Yes
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Reference toxicant within cusum chart limits
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140.3-243.6 mg/L
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223.1 mg/L
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Yes
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Test Report Authorised by Dr Rick Krassoi, Director on 7 November 2007
Results are based on the samples in the condition as received by ESA
NATA Accredited Laboratory Number: 14709
The tests, calibrations or methods covered by this document have been performed in accordance with NATA requirements which include the requirements of ISO/IEC 17025 and are traceable to Australian national standards of measurement.
Toxicity Test Report: TR0294/2
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Client:
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Green Mountain Solutions
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ESA Job #:
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PR0294
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|
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PO Box 1065
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Date Sampled:
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Not given
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|
|
Tweed Heads NSW 2485
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Date Received:
|
13 September 2007
|
|
Attention:
|
Mr Steve Hall
|
Sampled By:
|
Green Mt Solutions
|
|
Contract #:
|
n/a
|
Quote #:
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PI0294_q01
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Lab ID No.: 2320 Sample Name: Natra-Kleen Sample Description: Green liquid detergent
Test Performed: 72-hour growth inhibition (chronic) test using the freshwater unicellular
green alga Selenastrum capricornutum (Raphidocellus subcapitata)
Test Protocol: USEPA OPPTS 840.5400 and USEPA Method 1003.0
Deviations from Protocol: None. USEPA Media prepared without EDTA.
Source of Test Organisms: CSIRO Culture CS-327 (axenic laboratory culture)
Test Initiated: 7 October 2007 at 2030 hrs
Test Performed: 72-hour growth inhibition (chronic) test using the freshwater unicellular
green alga Selenastrum capricornutum (Raphidocellus subcapitata)
Test Protocol: USEPA OPPTS 840.5400 and USEPA Method 1003.0
Deviations from Protocol: None. USEPA Media prepared without EDTA.
Source of Test Organisms: CSIRO Culture CS-327 (axenic laboratory culture)
Test Initiated: 7 October 2007 at 2030 hrs
Summary of Test Method: The test temperature was 25 + 1 °C. The test vessels were 20mL borosilicate
glass scintillation vials with 20mL test solution. There were 4 replicate vessels per treatment. The initial cell
density was 10,000 cells/ mL, determined by haemocytometer counts. Cell densities were enumerated at 72
hours by haemocytometer counts. The test vessels were shaken by hand 2 times per day.
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Sample 2320: Natra-Kleen Concentration Cell density: (mg/L) 72 h
(mean ± SD) / mL (x 103)
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Vacant
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Vacant
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0 (control) 33.0 ± 3.2
3.13 35.3 + 2.5
6.25 32.3 + 3.9
12.5 13.3 + 2.2*
25 6.8 + 2.2*
50 2.0 ± 0.8*
72 hlC50= 11.3 (10.3-12.3)
mg/L
NOEC = 6.25 mg/L
LOEC = 12.5 mg/L
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•Significantly lower % cell yield compared with the control treatment (Dunnetts Test, 1-tailed, P=0.05)
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QA/QC Parameter
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Criterion
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This Test
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Criterion met?
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Control minimum cell density
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>20,000 / mL
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33,000/mL
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Yes
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Test Temperature limits
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25.0 ±1°C
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25.0°C
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Yes
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Reference Toxicant within cusum chart limits
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0.18-0.77 ugCu/L
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0.44
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Yes
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Test Report Authorised by: Dr Rick Krassoi, Director on 7 November 2007
Results are based on the samples in the condition as received by ESA
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