BIOTEST LABORATORIES PTY LTD
A.B.N. 82 010 924 571 Our Reference 153265
Unit 4, Cnr Smallwood & Darnick St.. Underwood
P.O. Box 4041 - Eight Miles Plains
Qld. 4113
Telephone (07) 3841 2232 Fax. (07) 3341 2241
14 September, 2007
Mr Steve hall
Green Mountain Solutions
Po Box 1065
Tweedheads NSW 2485
Dear Sir,
Re: CHALLENGE TEST - NATRA-KLEEN
A sample was tested as received at the laboratory on the 10th of September 2007.
This sample was analysed as directed on the 12th of September 2007 to evaluate the
performance of the product against the challenge organisms listed below.
The methodology employed followed guidelines outlined in the British Standard 3286.
The test condition and results are as follows which pertain only to the sample as tested;-
Sample Description : Natra-Kleen Biotest Sample No : 7253/21
Test Conditions
|
Temperature : Ambient
Contact Period 1, 5 and 10 minutes
Recovery Media Initial Diluent - Nutrient Broth with 3% Tween 80
Product Dilution Neat
Soil : Nil
|
|
Challenge Organisms
|
|
Initial Challenge
|
|
Escherichia coli
|
ATCC 8739
|
2.6x106
|
|
Pseudomonas aeruginosa
|
ATCC 9027
|
1.0x107
|
|
Staphylococcus aureus
|
ATCC 6538
|
2.0x106
|
|
Salmonella typhimurium
|
ATCC 13311
|
6.2x106
|
|
Listeria monocytogenes
|
ACM 98
|
1.3x107
|
BIOTEST LABORATORIES PTY LTD Our Ref.: 153265
TEST RESULTS
|
Challenge Organism
|
Recovery Count
|
|
1 minutes
|
Kill Factor
|
5 minutes
|
Kill Factor %
|
10 minutes
|
Kill factor %
|
|
E.coli
|
2.5x106
|
3.84
|
1.2X106
|
53.35
|
1.7x106
|
34.62
|
|
P. aeruginosa
|
3.7x105
|
63.00
|
1.5X106
|
85.00
|
2.5x106
|
75.00
|
|
S. aureus
|
1.8x107
|
0.00
|
7.9x104
|
96.05
|
1.0x105
|
95.00
|
|
S. typhimurium
|
5.3x106
|
14.52
|
3.7X106
|
40.32
|
5.0x106
|
19.35
|
|
L monocytogenes
|
5.4x106
|
70.00
|
4.7X106
|
73.89
|
8.4x106
|
53.33
|
|
Test Organism
|
Product Inactivation
|
|
Escherichia coli
|
Satisfactory at -1 dilution resulting in a sensitivity of <10.
|
|
Pseudomonas aeruginosa
|
Satisfactory at -1 dilution resulting in a sensitivity of <10.
|
|
Staphylococcus aureus
|
Satisfactory at -1 dilution resulting in a sensitivity of <10.
|
|
Salmonella typhimurium
|
Satisfactory at -1 dilution resulting in a sensitivity of <10.
|
|
Listeria monocytogenes
|
Satisfactory at -1 dilution resulting in a sensitivity of <10.
|
Test Method
Biotest Method 5.2. Following guidelines in the British Standard 3286:1960. The product was tested neat.
Discussion
A Kill Factor of 99.99% or greater is regarded as evidence of significant bactericidal action. (Kill factor is defined as the ratio of the initial to final count). The product as tested did not display significant bactericidal action against any organisms tested after 10 minutes contact time.
Yours faithfully,
BIOTEST LABORATORIES PTY. LTD.,
FIONA MCNICOL B.App.Sc (Biotechnology)
Microbiology Manager
|
